Combinatorial Optimization of Resveratrol Production in Engineered E. coli.
Identifieur interne : 000130 ( Main/Exploration ); précédent : 000129; suivant : 000131Combinatorial Optimization of Resveratrol Production in Engineered E. coli.
Auteurs : Ying Zhao [République populaire de Chine] ; Bi-Han Wu [République populaire de Chine] ; Zhen-Ning Liu [République populaire de Chine] ; Jianjun Qiao [République populaire de Chine] ; Guang-Rong Zhao [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2018.
Descripteurs français
- KwdFr :
- Acyl-carrier protein S-malonyltransferase (génétique), Acyl-carrier protein S-malonyltransferase (métabolisme), Corynebacterium glutamicum (génétique), Corynebacterium glutamicum (métabolisme), Escherichia coli (génétique), Escherichia coli (métabolisme), Génie métabolique (méthodes), Malonyl coenzyme A (génétique), Malonyl coenzyme A (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Resvératrol (métabolisme), Voies de biosynthèse (MeSH).
- MESH :
- enzymologie : Phanerochaete.
- génétique : Acyl-carrier protein S-malonyltransferase, Corynebacterium glutamicum, Escherichia coli, Malonyl coenzyme A, Phanerochaete.
- métabolisme : Acyl-carrier protein S-malonyltransferase, Corynebacterium glutamicum, Escherichia coli, Malonyl coenzyme A, Resvératrol.
- méthodes : Génie métabolique.
- Voies de biosynthèse.
English descriptors
- KwdEn :
- Acyl-Carrier Protein S-Malonyltransferase (genetics), Acyl-Carrier Protein S-Malonyltransferase (metabolism), Biosynthetic Pathways (MeSH), Corynebacterium glutamicum (genetics), Corynebacterium glutamicum (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Malonyl Coenzyme A (genetics), Malonyl Coenzyme A (metabolism), Metabolic Engineering (methods), Phanerochaete (enzymology), Phanerochaete (genetics), Resveratrol (metabolism).
- MESH :
- chemical , genetics : Acyl-Carrier Protein S-Malonyltransferase, Malonyl Coenzyme A.
- chemical , metabolism : Acyl-Carrier Protein S-Malonyltransferase, Malonyl Coenzyme A, Resveratrol.
- enzymology : Phanerochaete.
- genetics : Corynebacterium glutamicum, Escherichia coli, Phanerochaete.
- metabolism : Corynebacterium glutamicum, Escherichia coli.
- methods : Metabolic Engineering.
- Biosynthetic Pathways.
Abstract
Resveratrol, a plant-derived polyphenolic compound with various health activities, is widely used in nutraceutical and food additives. Herein, combinatorial optimization of resveratrol biosynthetic pathway and intracellular environment of E. coli was carried out. By screening pathway genes from various species and exploring their expression pattern, we initially constructed resveratrol-producing strains. Further targeting at availability of malonyl-CoA through expressing ACC of Corynebacterium glutamicum and antisense inhibiting native fabD significantly increased resveratrol biosynthesis. Transport engineering for resveratrol secretion and molecular chaperones helping for folding heterologous enzymes were employed to improve the intracellular environments in remarkable degrees. By introducing PcTAL of Phanerochaete chrysosporium and tuning expression model of PcTAL, At4CL, and VvSTS, an engineered E. coli produced 57.77 mg/L of resveratrol from l-tyrosine. After integrating the above strategies, resveratrol titer reached to 238.71 mg/L from l-tyrosine. The combinatorial optimization in this study provides a promising strategy to produce valuable natural products in heterologous expression systems.
DOI: 10.1021/acs.jafc.8b05014
PubMed: 30488696
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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xml:lang="fr">République populaire de Chine</country><wicri:regionArea>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 </wicri:regionArea><wicri:noRegion>Tianjin 300350 </wicri:noRegion></affiliation></author><author><name sortKey="Liu, Zhen Ning" sort="Liu, Zhen Ning" uniqKey="Liu Z" first="Zhen-Ning" last="Liu">Zhen-Ning Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 </wicri:regionArea><wicri:noRegion>Tianjin 300350 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , 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Technology , Tianjin University , Tianjin 300350 , China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 </wicri:regionArea><wicri:noRegion>Tianjin 300350 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 , China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 </wicri:regionArea><wicri:noRegion>Tianjin 300350 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</nlm:affiliation><country xml:lang="fr">République populaire de 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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 </wicri:regionArea><wicri:noRegion>Tianjin 300350 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 </wicri:regionArea><wicri:noRegion>Tianjin 300350 </wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acyl-Carrier Protein S-Malonyltransferase (genetics)</term><term>Acyl-Carrier Protein S-Malonyltransferase (metabolism)</term><term>Biosynthetic Pathways (MeSH)</term><term>Corynebacterium glutamicum (genetics)</term><term>Corynebacterium glutamicum (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Malonyl Coenzyme A (genetics)</term><term>Malonyl Coenzyme A (metabolism)</term><term>Metabolic Engineering (methods)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Resveratrol (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acyl-carrier protein S-malonyltransferase (génétique)</term><term>Acyl-carrier protein S-malonyltransferase (métabolisme)</term><term>Corynebacterium glutamicum (génétique)</term><term>Corynebacterium glutamicum (métabolisme)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Génie métabolique (méthodes)</term><term>Malonyl coenzyme A (génétique)</term><term>Malonyl coenzyme A (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Resvératrol (métabolisme)</term><term>Voies de biosynthèse (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Acyl-Carrier Protein S-Malonyltransferase</term><term>Malonyl Coenzyme A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyl-Carrier Protein S-Malonyltransferase</term><term>Malonyl Coenzyme A</term><term>Resveratrol</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Corynebacterium glutamicum</term><term>Escherichia coli</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acyl-carrier protein S-malonyltransferase</term><term>Corynebacterium glutamicum</term><term>Escherichia coli</term><term>Malonyl coenzyme A</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Corynebacterium glutamicum</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Metabolic Engineering</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acyl-carrier protein S-malonyltransferase</term><term>Corynebacterium glutamicum</term><term>Escherichia coli</term><term>Malonyl coenzyme A</term><term>Resvératrol</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génie métabolique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biosynthetic Pathways</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Voies de biosynthèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Resveratrol, a plant-derived polyphenolic compound with various health activities, is widely used in nutraceutical and food additives. Herein, combinatorial optimization of resveratrol biosynthetic pathway and intracellular environment of E. coli was carried out. By screening pathway genes from various species and exploring their expression pattern, we initially constructed resveratrol-producing strains. Further targeting at availability of malonyl-CoA through expressing ACC of Corynebacterium glutamicum and antisense inhibiting native fabD significantly increased resveratrol biosynthesis. Transport engineering for resveratrol secretion and molecular chaperones helping for folding heterologous enzymes were employed to improve the intracellular environments in remarkable degrees. By introducing PcTAL of Phanerochaete chrysosporium and tuning expression model of PcTAL, At4CL, and VvSTS, an engineered E. coli produced 57.77 mg/L of resveratrol from l-tyrosine. After integrating the above strategies, resveratrol titer reached to 238.71 mg/L from l-tyrosine. The combinatorial optimization in this study provides a promising strategy to produce valuable natural products in heterologous expression systems.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30488696</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>66</Volume><Issue>51</Issue><PubDate><Year>2018</Year><Month>Dec</Month><Day>26</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Combinatorial Optimization of Resveratrol Production in Engineered E. coli.</ArticleTitle><Pagination><MedlinePgn>13444-13453</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.8b05014</ELocationID><Abstract><AbstractText>Resveratrol, a plant-derived polyphenolic compound with various health activities, is widely used in nutraceutical and food additives. Herein, combinatorial optimization of resveratrol biosynthetic pathway and intracellular environment of E. coli was carried out. By screening pathway genes from various species and exploring their expression pattern, we initially constructed resveratrol-producing strains. Further targeting at availability of malonyl-CoA through expressing ACC of Corynebacterium glutamicum and antisense inhibiting native fabD significantly increased resveratrol biosynthesis. Transport engineering for resveratrol secretion and molecular chaperones helping for folding heterologous enzymes were employed to improve the intracellular environments in remarkable degrees. By introducing PcTAL of Phanerochaete chrysosporium and tuning expression model of PcTAL, At4CL, and VvSTS, an engineered E. coli produced 57.77 mg/L of resveratrol from l-tyrosine. After integrating the above strategies, resveratrol titer reached to 238.71 mg/L from l-tyrosine. The combinatorial optimization in this study provides a promising strategy to produce valuable natural products in heterologous expression systems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Bi-Han</ForeName><Initials>BH</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Zhen-Ning</ForeName><Initials>ZN</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiao</LastName><ForeName>Jianjun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Guang-Rong</ForeName><Initials>GR</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6881-9042</Identifier><AffiliationInfo><Affiliation>Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Systems Bioengineering , Ministry of Education , Tianjin 300350 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>SynBio Research Platform , Collaborative Innovation Center of Chemical Science and Engineering , Tianjin 300350 , China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>524-14-1</RegistryNumber><NameOfSubstance UI="D008316">Malonyl Coenzyme A</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.39</RegistryNumber><NameOfSubstance UI="D051080">Acyl-Carrier Protein S-Malonyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>Q369O8926L</RegistryNumber><NameOfSubstance UI="D000077185">Resveratrol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D051080" MajorTopicYN="N">Acyl-Carrier Protein S-Malonyltransferase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053898" MajorTopicYN="N">Biosynthetic Pathways</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048230" MajorTopicYN="N">Corynebacterium glutamicum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008316" MajorTopicYN="N">Malonyl Coenzyme A</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060847" MajorTopicYN="N">Metabolic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000077185" MajorTopicYN="N">Resveratrol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Escherichia coli</Keyword><Keyword MajorTopicYN="N">combinatorial optimization</Keyword><Keyword MajorTopicYN="N">intracellular environment</Keyword><Keyword MajorTopicYN="N">metabolic engineering</Keyword><Keyword MajorTopicYN="N">resveratrol</Keyword><Keyword MajorTopicYN="N">synthetic biology</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30488696</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.8b05014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Ying" sort="Zhao, Ying" uniqKey="Zhao Y" first="Ying" last="Zhao">Ying Zhao</name></noRegion><name sortKey="Liu, Zhen Ning" sort="Liu, Zhen Ning" uniqKey="Liu Z" first="Zhen-Ning" last="Liu">Zhen-Ning Liu</name><name sortKey="Liu, Zhen Ning" sort="Liu, Zhen Ning" uniqKey="Liu Z" first="Zhen-Ning" last="Liu">Zhen-Ning Liu</name><name sortKey="Liu, Zhen Ning" sort="Liu, Zhen Ning" uniqKey="Liu Z" first="Zhen-Ning" last="Liu">Zhen-Ning Liu</name><name sortKey="Qiao, Jianjun" sort="Qiao, Jianjun" uniqKey="Qiao J" first="Jianjun" last="Qiao">Jianjun Qiao</name><name sortKey="Qiao, Jianjun" sort="Qiao, Jianjun" uniqKey="Qiao J" first="Jianjun" last="Qiao">Jianjun Qiao</name><name sortKey="Qiao, Jianjun" sort="Qiao, Jianjun" uniqKey="Qiao J" first="Jianjun" last="Qiao">Jianjun Qiao</name><name sortKey="Wu, Bi Han" sort="Wu, Bi Han" uniqKey="Wu B" first="Bi-Han" last="Wu">Bi-Han Wu</name><name sortKey="Wu, Bi Han" sort="Wu, Bi Han" uniqKey="Wu B" first="Bi-Han" last="Wu">Bi-Han Wu</name><name sortKey="Wu, Bi Han" sort="Wu, Bi Han" uniqKey="Wu B" first="Bi-Han" last="Wu">Bi-Han Wu</name><name sortKey="Zhao, Guang Rong" sort="Zhao, Guang Rong" uniqKey="Zhao G" first="Guang-Rong" last="Zhao">Guang-Rong Zhao</name><name sortKey="Zhao, Guang Rong" sort="Zhao, Guang Rong" uniqKey="Zhao G" first="Guang-Rong" last="Zhao">Guang-Rong Zhao</name><name sortKey="Zhao, Guang Rong" sort="Zhao, Guang Rong" uniqKey="Zhao G" first="Guang-Rong" last="Zhao">Guang-Rong Zhao</name><name sortKey="Zhao, Ying" sort="Zhao, Ying" uniqKey="Zhao Y" first="Ying" last="Zhao">Ying Zhao</name><name sortKey="Zhao, Ying" sort="Zhao, Ying" uniqKey="Zhao Y" first="Ying" last="Zhao">Ying Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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